![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1916108:Design
Designed by: Jonathan Chen Group: iGEM16_UC_Davis (2016-10-12)
Ara-Inducible Osc7112_5903g3 Expression System
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 131
Illegal SpeI site found at 815 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 744
Illegal SpeI site found at 815 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal BamHI site found at 791 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 131
Illegal SpeI site found at 815 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 131
Illegal SpeI site found at 815
Illegal NgoMIV site found at 1311
Illegal AgeI site found at 454
Illegal AgeI site found at 1401 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.
Source
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.